The launch video teases a number of the game's villains, including Rhino, Iguana and Vermin.
The Amazing Spider-Man is available now in North America and later this week in Europe. It releases ahead of the movie of the same name, starring Andrew Garfield, Emma Stone and Rhys Ifans.
The game is developed by Beenox Studios, the team behind Spider-Man: Shattered Dimensions and Spider-Man: Edge of Time. Its debut trailer was shown at the Spike Video Game Awards in December 2011.
Spider-Man creator Stan Lee will appear as a playable character for users who pre-order the title from GAME.
'The Stan Lee Adventure Pack' features a mission where the writer must collect pages of his latest script using Spider-Man's abilities.
The Amazing Spider-Man movie will be released on 3D, IMAX and conventional screens on July 3 worldwide.
Screenshot gallery: View The Amazing Spider-Man Iguana images below: