The 'Wimoweh' gameplay trailer is set to the music of 'The Lion Sleeps Tonight'.
It showcases the abilities of the game's four playable characters and some of the opposition they face in battle.
People who pre-order the game from selected retailers will gain access to the Borderlands 2 Premiere Club, the contents of which are advertised at the end of the trailer.
It includes a Gearbox Gun Pack featuring unique weaponry, a Golden Key to open a rare item in the Golden Sanctuary Loot Chest, and a Vault Hunter's Relic to boost gear-hunting pick-ups when exploring.
Borderlands 2 will be available for Xbox 360, PlayStation 3 and PC on September 18 in North America and on September 21 across Europe.
> Read Digital Spy's preview of Borderlands 2
> Read Digital Spy's Borderlands 2 interview with Gearbox Software
Watch the Borderlands 2 Doomsday trailer below: