Originally scheduled to release alongside its console counterparts, the PC version was delayed in June. As speculated, it will now launch on November 23.
Assassin's Creed 2, Brotherhood and Revelations all suffered similar delays.
Officially unveiled in March, Assassin's Creed 3 will take place over a 30-year period in colonial America beginning in 1777.
It features a brand new lead named Connor, the offspring of an English father and Native American mother.
PlayStation Vita spinoff Assassin's Creed 3: Liberation was announced at this year's E3 Gaming Expo.
It will support cross-play with the PlayStation 3 edition of Assassin's Creed 3, allowing players to unlock bonuses in both games by trading goods.
Assassin's Creed 3 and Liberation will be released for Xbox 360, PlayStation 3 and PlayStation Vita on October 31 worldwide. A Wii U version is also in development and will be available during the system's launch window this holiday season.
The title is on course to break Ubisoft's record for the most pre-ordered game of all time.
> 'Assassin's Creed 3' E3 2012 preview: Eight anticipated features
> Read our 'Assassin's Creed 3' interview with Ubisoft
Watch a trailer for Assassin's Creed 3 below: