The Andrew Garfield-led superhero reboot pulls in more than £11 million to easily coast past its competition and secure the number one spot.
Amazing Spider-Man's opening total, which includes previews from Tuesday, Wednesday and Thursday, is more than the rest of the top ten combined and the second-highest UK debut of the year behind Avengers's £15.78 million haul.
Ice Age 4: Continental Drift falls down a place to number two, while The Five-Year Engagement holds its spot at number three.
Katy Perry's 3D concert movie Part of Me lands at number four with £449,491 from its 326 locations. The documentary's total falls short of last year's Justin Bieber: Never Say Never, which took £821,000 on its opening weekend, but outpaces Glee: The 3D Concert Movie's £356,271 from last August.
Elsewhere, the only other new release to make it into the top ten is Bollywood film Bol Bachchan at number eight.
The UK box office top ten in full:
1. (-) The Amazing Spider-Man - £11,091,972
2. (1) Ice Age 4: Continental Drift - £719,769
3. (3) The Five-Year Engagement - £514,702
4. (-) Katy Perry: Part of Me - £449,491
5. (4) Men in Black 3 - £389,853
6. (2) Prometheus - £324,779
7. (5) Snow White and the Huntsman - £312,318
8. (-) Bol Bachchan - £160,941
9. (7) Friends with Kids - £134,752
10. (10) Killer Joe - £122,831
Watch Digital Spy interview the stars of The Amazing Spider-Man below: