The new clip, titled 'Bride', premiered online earlier today and follows the star's first fashion video in the series, which premiered last week.
GaGa will release six videos in total, with each one centring around a character from her original 'You and I' music video.
Announcing the release of the clip on Twitter, GaGa said: "The second instalment of the HAUS of Ü. In collaboration with INEZ+VINOODH, here is "BRIDE" (sic)."
The new video sees GaGa rip the wig off her head and massage her breasts with a robotic hand, while rubbing dirt over her body.
It was recently revealed that Wild Beasts and Metronomy's remixes of GaGa's 'You and I' will feature on a special edition picture disc of the single.
Lady GaGa's 'You and I' will receive its official release on September 18.
Watch the second alternative version of Lady GaGa's 'You and I' video titled 'Bride' below: