The track clocks a fourth week at number one, followed by Carly Rae Jepsen's 'Call Me Maybe' at two and Maroon 5's 'Payphone' at three.
Meanwhile, Brittany Cairns lands at seven with 'Different Worlds', Rihanna slips from seven to nine with 'Where Have You Been', and Delta Goodrem holds at ten with 'Sitting On Top Of The World'.
Further down the chart, Rebecca Ferguson re-enters at 14 with 'Nothing's Real But Love', Jes Hudak rebounds at 21 with 'Different Worlds', and Skrillex lands at 27 with 'Make It Bun Dem'.
Coldplay and Rihanna's 'Princess of China' debuts at 33, and Rita Ora starts at 41 with 'How We Do (Party)'.
The Top 10 singles in full (click where possible for our reviews):
1. (1) Flo Rida: 'Whistle'
2. (2) Carly Rae Jepsen: 'Call Me Maybe'
3. (3) Maroon 5 ft. Wiz Khalifa: 'Payphone'
4. (5) Ed Sheeran: 'Lego House'
5. (6) Pitbull: 'Back In Time'
6. (4) Nicki Minaj: 'Starships'
7. (*) Brittany Cairns: 'Different Worlds'
8. (8) Florence + the Machine: 'Never Let Me Go'
9. (7) Rihanna: 'Where Have You Been'
10. (10) Delta Goodrem: 'Sitting On Top Of The World'
Watch Flo Rida's 'Whistle' music video below: