The couple collaborated for a cover of 'Telephone', which they recorded especially for their daughter Eve's 21st birthday.
Bono unveiled his cover of the song at a party in Las Vegas on Saturday (July 7), with guests including Interscope Records boss Jimmy Iovine, film director Michael Bay and basketball player Kobe Bryant.
The Irish rocker introduced the track to attendees at 1Oak at the Mirage, as well as having two magnums of Dom Perignon champagne delivered to his daughter's table, reports the New York Post.
Lady GaGa and Beyoné's original version of 'Telephone' reached number one on the UK singles chart and number three on the US Billboard 200.
Meanwhile, Bono recently revealed that U2's latest recording sessions are among the band's best ever.
Watch the music video for Lady GaGa and Beyoncé's 'Telephone' below: