The singer, born Leo Ihenacho and a former singer with The Streets, started at the London Eye on July 11 at 9pm before moving on to the London Dungeons, a South London prison cell, Smithfield's meat market and other venues.
Ihenacho's charity 24:24 tour closed with a finale performance at London's Funky Buddha Club, taking to the stage on July 12 before 8pm.
"Morning lions... I dont know why im up... But i cant sleep," he said on Twitter after completing the shows.
"Thanx to all that came to the finale of #leo24gigs it was massive... "
Fans can continue to donate to The Prince's Trust via the Vodafone JustTextGiving service by texting LION82 £3 to 70070.
Fundraisers can get their own personalised text code from the Vodafone JustTextGiving page.
Watch Leo the Lion perform at the offices of Zoo magazine at hour 18 of his epic run of shows below: