The pilot follows the men and women of the Chicago Fire Department, with Kinney playing Squad Lieutenant Kelly Severide - the leader of the elite firefighters - reports Deadline.
Written by Michael Brand and Derek, the pilot will be directed by Jeffrey Nachmanoff, who has previously helmed episodes of Homeland.
Law & Order's Dick Wolf will serve as one of the producers on the show.
Kinney was previously cast in NBC's A Man's World and starred in the medical series Trauma.
He is currently rumoured to be in a relationship with Lady GaGa. The couple reportedly started dating after Kinney starred in the singer's 'You and I' music video.
Watch the music video for Lady GaGa's 'You and I' below: